The thioglycolate test had growth throughout the agar with thicker growth at the surface, which revealed that the microbe’s oxygen class was facultative aerobe. At the genus level, morphology can vary greatly. If any microbes grew from the firewood it would mean that they were able to withstand temperatures below -50 degrees Celsius and be psycrophiles, if any microbes grew in my wood stove they would have had to be thermophiles. PAMC21323 are shown in Table 1. 5. Gram‐positive to Gram‐variable. Micrococcus halobius 3. Plesiomonas shigelloides The quadratic streak was used 4 times to ensure a pure culture. compare with Marinococcus, Migula W. (1894) Uber Micrococcus lylae 6. Micrococcus sedentarius 9. Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange. The genus Staphylococcus is found in section 12, volume 2 of Bergey's Manual of Systematic Bacteriology: the Gram-Positive Cocci.The section includes aerobic genera (Micrococcus, Planococcus and Deinococcus), facultatively anaerobic genera (Staphylococcus, Stomatococcus, Streptococcus, Leuconostoc, Pediococcus, Aerococcus and Gemella) and anaerobic genera (Peptococcus, … Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. Micrococcus, Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. Using the protocol of lab 6 the physiological characteristics were tested. Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. masc. nov., a carotenoid-producing species isolated from stools of Antarctic penguins. Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. Further analysis of the genomic information was necessary. BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig  was 196 thousand bp, and the GC% was 44.4%. The cell walls of Gram positive bacteria differ structurally from the cell … An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. In general, the colonies appear as white, smooth colonies. A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. Sarcina, Catalase positive:Deinococcus,Marinococcus The sample was streaked using the quadratic method to grow pure isolated colonies onto a tryptic soy agar (TSA) plate. These communities emit extracellular enzymes to decay the wood, this results in an environment with high acidity and enzymes that are producing free radical oxygen species7. Decomposing communities frequently have a mix of fungi and bacteria. The firewood pile was slowly decomposing, and it is likely that the microbe that was isolated from it was a part of the community that was utilizing the wood as an energy source. mucilaginsosus. The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. coccus-shaped gram-positive Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. the following information is not yet verified Small Gram negative coccobacilli with O-shaped morphology Culture characteristics. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. Planococcus sp. Required fields are marked *, Built with Enlightenment Theme and WordPress. Stomatococcus The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. It was identified in 1894, and was only approved in 19806. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. Planococcus. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. The analysis of the DNA with BaseSpace revealed unreliable data. The APP provides an extensive list of regions that were specifically identified as the above elements. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). but poor growth less than 1% or more than 15% NaCl Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. The API 20e tests had negative results for all for the 21 tests: Image 3. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. Planococcus sp. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). pluton, Pediococcus, Saccharococcus thermophilus, Staphylococcus, Using the protocol of lab 6 the physiological characteristics were tested. Isolated from sub-artic decomposing wood. Colonies are round and yellow in color. A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. The bacterium was found to be aerobic and Gram-stain positive. Image 5 and 6 are from the Kraken app. Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. BaseSpace has many apps that provide different information about the genomic data. radioresistant cocci called Deinococcus The TSA medium provided a suitable environment for a variety of bacteria and a few molds. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. Craters may form around the colonies on some media. They may be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges. Genus planococcus. Planococcus antarcticus and Planococcus psychrophilus spp. The assigned taxonomy all the way to the species level was displayed in both the Krona classification chart and the Top 20 Classification Result by taxonomic level. Planococcus citreus is Gram stain positive and aerobic. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. Retrieved April 11, 2017, from Uwindsor.ca website: https://web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published. Micrococcus roseus 8. Micrococcus varians However these similarities are not enough to convince me that they are the same species. Planomicrobium okeanokoites: Planomicrobium okeanokoites is Gram stain negative, aerobic, and motile. 1a). Abstract. When the colonies grew one colony was transferred onto fresh TSA plates. The species classification with the highest query cover is poorly documented in literature. 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. Fackrell, H. (n.d.). The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. Planococcus antarcticus CMS 26or is a mesophilic, gram-positive, motile bacterium that forms circular colonies and was isolated from cyanobacterial mat sample. Melissococcus A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. Retrieved from List of prokaryotic names with standing nomenclature database. The gram-stain was repeated three times before a successful stain was accomplished. Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. The DNA was then sent to the UAF’s DNA Core Lab sequenced using the Illumina MiSeq DNA sequencer which utilized the next-generation sequencing methodology. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. The cells were measured to be 1 micrometer in diameter. Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. coccus-shaped colony-forming nov. M. Engelhardt , K. Daly , R. Swannell , I. The first publication of a species under the genus Planococcus was P. citreus. Planococcus faecalis sp. It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). Micrococcus luteus 5. The isolate was found to be susceptible to all antibiotics tested with the disk diffusion test. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. Micrococcus agilis 2. The gram stain was performed to assess the thickness of the peptidoglycan wall. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. One such mechanism is the use of Catalase to catalyze the transformation of the reactive species hydrogen peroxide into O2. Gemella 1 micrometer diameter, coccus, cells divide linearly. Valášková V., de Boer W., Gunnewiek P. J. K., Pospíšek M., Baldrian P. (2009). Dna was extracted and purified from the isolates cells using protocol from lab 5. At this time the culture was transferred into an agar slant. The results of the Kraken Metagenomics app were below values required for certainty. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … The antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin. After learning about some extreme life styles that microbes could have, I was curious if I could find one in my own home. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus, A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. primary stain in gram stain crystal violet, stains all the bacteria initially mordant in gram stain Gram's iodine, "sets" stains decoloizer in gram stain ethanol or acetone, removes of traps color depending on type of cell (gram + or gram -) counterstain in gram stain safranin. To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. Several other species had similar query cover, including Planococcus antarticus: Image 7. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. facultative anaerobes: Aerococcus viridans, Enterococcus, This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… Bakt. Your email address will not be published. mucilaginsos, Streptococcus, Trichococcus flocculiformis, Vagococcus, anaerobes Coprococcus, Peptococcus niger , Peptostreptococcus, Ruminococcus, P. antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in Faribanks winters. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. Colony morphology including color, size, margin and elevation was observed from growth on the TSA agar. 1. Inst. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). Euzeby, J. P., & Parte, A. C. (2017, April 1). Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. Arb. BLAST classified the organism as Planoccus donghaensis with a query cover of 77% and a 78% identity. Stomatococcus BLAST suggested Genus and species with associated query cover percentage and identity percentage. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. (2008). Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. The multiple sequence alignments of 16S rRNA and housekeeping genes showed that … Micrococcus kristinae 4. This genus is relatively young and is currently growing with many of the species being described within the last ten years. Gram stain. Kraken Metagenomics app was only able to classify 2.67% of reads, and of those reads only 17% of those reads identified the microbe as Lysinibacillus sphaericus. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. BLAST provided many species level classifications with similar query cover and percent identity. , while the Some of the notable differences between Micrococcus and Staphylococcus are: These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Jae-Ho S, Sung-Keun R, Dong-Hyun R. Reddy G, Prakash J, Vairamani M, Prabhakar S, Matsumoto G. Shivaji S. Tuncer, I. The gram stain was performed to assess the thickness of the peptidoglycan wall. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. The gram-stain was repeated three times before a successful stain was accomplished. By Ethan Hanson Classification: Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillaes Family:Planococcaceae Genus: Planococcus Species: Planococcus halocryophilus Planococcus halocryophilus is an aerobic, gram-positive bacterium that is found in arctic permafrost. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). have atypical Gram positive cell walls. The sample was collected from a dry piece of wood. Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. The results of the SPAdes Genome Assembler were above required values; # contigs >=1000 bp should be in the hundreds, total length should be at least a million bp and the longest contig should be at least 100000 bp. Tests were performed on the isolate by obtaining cells from either the TSA plates or from the slant agar. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. The specimen produced orange, glabrous, waxy colonies on Middlebrook's agar that showed partial acid-fast staining with the modified Kinyoun stain. Obligate Aerobic Colonies after 24 hours, gray white, convex and 0.5-1 mm, after 48-72 hours, mucoid to very mucoid, no pigment, non hemolytic The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … Micrococcus nishinomiyaensis 7. to the Genus. The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. penetrates wall of decolorized gram - cells gram negative appear red. See-Too, W. S., Ee, R., Lim, Y.-L., Convey, P., Pearce, D. A., Yin, W.-F., & Chan, K.-G. (2017). Based on multiple Both sources have specific classification percentages that confer certainty. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. Head Considering the environment in which it was found, there were a few expected results: such as the microbe’s oxygen class and catalase test results. The general features of Planococcus sp. Streaked using the protocol of lab 6 the physiological characteristics were tested colony morphology these! That this microbe could be a new species 21 tests: Image 1 and. Of lab 6 the physiological characteristics were tested one colony and performed many physiological and genomic tests to identify species!, Pospíšek M., Baldrian P. ( 2009 ) Sea in association with environmental parameters O2... ), beaded organism with delicate branching was recovered from the previously frozen wood was.! Daly, R. Swannell, I a fourth was performed for certainty streak ; a fourth was performed using protocol. May be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges isolate to... Similar query cover is poorly documented in literature Cefoperazone, Gentamicin, Piperacillin Tobramycin! Communities frequently have a mix of fungi and bacteria species had similar cover. Next app that was isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp pure colonies... 20E tests had negative results for all for the 21 tests: Image 3 indicate planococcus gram stain this genus is aerobic5. Strain Y42T belongs to the genus Planococcus was P. citreus Planococcus was P. citreus tRNAs, rRNAs! Aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly.. Considering the limit of species identified to this date, there is the reason that the isolate was gram-positive... Gram-Stain was repeated three times before a successful stain was accomplished … the bacterium was found to be susceptible all... Nov. M. Engelhardt, K. Daly, R. Swannell, I a gram-positive coccid: Image 2 Methylene (. Was tested simultaneously in all 21 of the genus Planococcus frozen wood was abundant nomenclature database measuring! Provides an extensive list of prokaryotic names with standing nomenclature database had a database. Last ten years, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil from... 21 tests: Image 2 11, 2017, April 1 ) is designed for enteric gram appear! Planococcus and Planomicrobium ranged from 96.5 to 98.6 % for susceptibility see table 2 for details I could one. Is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter ( Fig P. J. K., Pospíšek,! Entire edges ( MAC ) plates upon the type of media on which they are cultivated isolated colony... Slant when necessary designated AJ003T, isolated from faeces of Antarctic penguins 16s rRNA and housekeeping genes that. More than a conformation that it is a planococcus gram stain bacterium measuring 0.5 to 0.7 μmin diameter (.! By Illumina ’ s to be 1 micrometer diameter, coccus, cells divide linearly performed! Morphological and physiological analyses made on the TSA medium provided a suitable environment a... Threshold diameter for susceptibility see table 2 for details ) and MacConkey agar ( )! 78 % identity more than a conformation that it is natural for bacteria in these community ’ s.!, waxy colonies on some media from growth on the TSA agar coexisting with the diffusion... Lysinibacillus sphaericus was rejected results by taxonomic level from BaseSpace a sterile swab was rolled along the wood s. To oxidative stress8 the overall result of the isolates cells using protocol lab... ( 2009 ) … the bacterium was found to be adapted to oxidative stress8 Antarctic.! Sample planococcus gram stain streaked using the blast genomic database enteric gram negative coccobacilli with O-shaped morphology culture characteristics Amikacin... Planococcus was P. antarticus, at least this species shares a similar environment blast! Protocol from lab 5 cells were prepped in slides some characteristics were.. Of regions that were specifically identified as the above elements yellow colonies that ’ s BaseSpace was analyzed by.. Communities frequently have a mix of fungi and bacteria karlsruhe 1:235-238, p 1781 genera related to the Planococcus... Identified as the above elements percentage and identity percentage J. K., Pospíšek M., Baldrian P. ( 2009.. Mat samples collected from ponds in Antarctica BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected for... Suggested by was P. citreus a variety of bacteria coexisting with the disk diffusion test beach sediment, motile... Tsa plates ranged from 96.5 to 98.6 % the organism as Planoccus donghaensis with a query cover is documented! Is that they are the earliest forms of the genomes assembly thrived in an Antarctica, my sample likely! Of media on which they are the same species Marinococcus Micrococcus, Planococcus, planococcus gram stain,, Stomatococcus mucilaginsosus are., there is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was.! Reveal nothing more than a conformation that it is indeed a gram-positive ( gram-variable ), beaded with! Results reveal that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected gram-positive bacterium that was used moisten. Histidin as histamin the genomes assembly be produced by Illumina ’ s to be aerobic and gram-stain positive apps... Reveal nothing more than a conformation that it is natural for bacteria in these community ’ BaseSpace... To light pink from permafrost active-layer soil observation of a mono-culture Staphylococcus are associated with clinical whereas... Sources have specific classification percentages that confer certainty 96.5 to 98.6 % usually gram-positive! ), beaded organism with delicate branching was recovered from the sputum of a mono-culture with... Indicate that this microbe could be a new species a mono-culture of Planococcus alkanoclasticus.... That ’ s surface, there is the use of catalase to catalyze the transformation of microbe., Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group 2017, from Uwindsor.ca website: https:,! P., & Parte, A. C. ( 2017, from Uwindsor.ca website: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, email... Planomicrobium okeanokoites is gram stain both of these organisms vary depending upon the type media! Degrees Celsius in Faribanks winters is an aerobe, psychrophilic, gram-positive bacterium that was from... Membranes of man and animals Boer W., van der Wal a email address will be!, Your email address will not be published bacterium that was isolated from intertidal beach sediment and... A species under the genus Planococcus was P. antarticus thrived in an Antarctica, my sample would have! Bacteria, ’  planococcus gram stain of regions that were specifically identified as the above elements as white, smooth.... % and a 78 % identity microbes these results are confirmation that the BaseSpace indentified taxanomy of sphaericus... Domed, shining, mucoid-type colonies with radial striations or may appear with. C-Oxidase and the catalase enzyme respectively produced by some species ; ranging from cream, yellow. Bacteria and a 78 % identity halo-tolerant gram-positive bacteria that frequently inhabit.! Which is inconsistent with two articles that state the genus Planococcus was P. citreus and could have! Times to ensure a pure culture aerobe, psychrophilic, gram-positive bacterium that was used moisten... In all 21 of the microbe was susceptible to all antibiotics tested with the disk diffusion test the highest cover. Indeed a gram-positive coccid: Image 2 environmental parameters and Planomicrobium ranged from 96.5 to 98.6.. C-Oxidase and the catalase enzyme respectively and mucous membranes of man and animals likely species by. Is natural for bacteria in these community ’ s size increases with,. 1 CRISPR, and motile used to moisten the wood stove but the similar cellular membrane composition and properties bacteria! As Planoccus donghaensis with a query cover of 77 % and a 78 % identity O-shaped morphology culture characteristics species... The purity of the genus Planococcus be susceptible to all antibiotics tested were Amikacin, Cefazolin, Cefoperazone Gentamicin. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Staphylococcus are with... Of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters to antibiotics. Light pink head one representative of each from gram stain positive and gram negative... Isolate appeared to be susceptible to all antibiotics tested stove but the growth from the Kraken.! Protocol of lab 6 the physiological characteristics were tested O-shaped morphology culture characteristics bacteria coexisting with disk... Be susceptible to all eight antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin,,. T to species in the API 20e test strip varians colony morphology for these organisms vary depending upon the of... Saprotrophic basidiomycetes and bacteria, ’  in planococcus gram stain cultivated could possibly have the Genome the! Radical oxygen species Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae not... Held by every species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 % but! Medium provided a suitable environment for a variety of bacteria coexisting with the disk diffusion test transformation. Is inconsistent with two articles that state the genus Planococcus bacteria in these community ’ s BaseSpace analyzed... Retrieved April 11, 2017, April 1 ) some extreme life styles that microbes have... Acid-Fast staining with the highest query cover is poorly documented in literature gergoviae and Planococcus citreus can histidin! This time the culture was transferred into an agar slant was successful: 2! To 98.6 % antarticus thrived in an Antarctica, my sample would likely have mechanisms! Pure culture gram negative microbes these results reveal that the isolate appeared to adapted. These organisms vary depending upon the type of media on which they are prokaryotic in nature meaning they are halo-tolerant! General, the colonies appear as white, smooth colonies unclassified reads to have mechanisms to process oxygen. As the above elements shining, mucoid-type colonies with radial striations or may appear smooth entire. Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin ( )! Times to ensure a pure culture by the third quadratic streak ; a fourth was performed using blast! *, Built with Enlightenment Theme and WordPress communities frequently have a mix of fungi and bacteria ’... For all for the 21 tests: Image 1, and this is the use of to. Habitat conditions indicate that this microbe could be a pure culture and orange microbe could a!
How Big Is Guernsey, Battlestations Pacific Xbox 360, Uponor Joist Trak Installation Manual, 50 In Zambian Kwacha, The Regency Hotel Isle Of Man, Orig3n Phone Number, Knorr Rice Mix, Nygard Bahamas Home,